Fig. 1.

Serum concentration influences the effects of ANE on cell morphology and various proteins. OC2 or the indicated SAS cells cultured with various concentrations of FBS were treated with 0.5 mg/ml ANE for 24 h. Cells were then directly photographed (A) or stained with AO/EtBr for evaluating membrane damage (B). The other panels of cells were harvested for monitoring the changes of indicated proteins by Western blot (C, D). The results are the representative figures from at least two independent experiments.