Fig. 2.

ANE under lower serum condition induces inflammatory cytokines more efficiently. (A) OC2 cells cultured with indicated concentrations of FBS were treated with 0.5 mg/ml ANE. The cells were harvested for measurement of IL6, IL8, RANTES or VEGF transcripts 24 h later. The value of the untreated, 10% FBS-supplemented cells was defined as 1. (B) The cells transfected with IL8 or COX2 promoter reporter were cultured in medium containing 1% FBS. The luciferase activity was determined 24 h after ANE treatment. (C) Similarly treated cells were harvested for Western blot. The upper signal marked with asterisk might be a glycosylated form of MCP1.