Fig. 4.

Detection of apoptosis TUNEL assay in the liver tissue of rats. The presence of apoptosis after treatment with (2, 10 and 50 mg/kg/ b.wt) of 4-NP by intraperitoneal injection for 30 days. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

(B–D) DNA fragmentation, characteristic of apoptosis (blacks arrows), (red arrows) indicates ballooning. (E) The graph representing average number of TUNEL-positive cells in each group). Observations done at 200× magnification. Apoptotic index of liver (%). The tissue sections were counted for each rat. The symbol represents statistical significant (ANOVA) from control: *p < 0.05, **p < 0.01, ***p < 0.001.