REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD45 30-F11 1 in 400 Mouse	eBioscience	Cat: 45-0451-82; RRID: AB_1107002
TER119 Ter-119 1 in 400 Mouse	eBioscience	Cat:12-5921-82; RRID: AB_466042
CD31 390 1 in 400 Mouse	eBioscience	Cat: 25-0311-82; RRID: AB_469616
Endomucin V.7C7 1 in 100 Mouse	Santa Cruz Biotechnology	Cat: sc-65495; RRID: AB_2100037)
Sca-1 D7 1 in 400 Mouse	eBioscience	Cat: 17-5981-83 RRID: AB_469488
Lin - 1 in 400 Mouse	Biolegend	Cat: 133311; RRID: AB_11203535
Kit 2B8 1 in 400 Mouse	BD Bioscience	Cat: 553354; RRID: AB_394805
CD48 HM48-1 1 in 400 Mouse	Biolegend	Cat: 103432; RRID: AB_256163
CD150 TC15-12F12.2 1 in 400 Mouse	Biolegend	Cat: 115914; RRID: AB_439797
IB4 - 100ug/mouse Mouse	Life Technology	Cat: I21411; RRID: AB_2314662
Sca-1/Ly6A/E EPR3355 1 in 100 Mouse	Abcam	Cat: ab109211; RRID: AB_10862573
Ki67 SolA15 2 in 100 Mouse	eBioscience	Cat: 12-5698-82; RRID: AB_11150954
Ki67 20Raj1 2 in 100 Human	eBioscience	Cat: 50-5699-82; RRID: AB_2574237
rAnnexin V - 2 in 100 Mouse/Human	eBioscience	Cat: BMS306; RRID: AB_2687874
CD45 PD7/26 + 2B11 1 in 100 Human	Dako	Cat: M0701; RRID: AB_2314143
CD34 581 1 in 25 Human	BD Bioscience	Cat: 555821; RRID: AB_396150
CD45 2D1 1 in 25 Human	BD Bioscience	Cat: 47-0459-42; RRID: AB_1944368
CD38 HIT2 1 in 25 Human	BD Bioscience	Cat: 555462; RRID: AB_1994368
Ki67 20Raj1 1 in 25 Human	eBioscience	Cat: 50-5699; RRID: AB_2574237
CD45 HI30 20ug/mouse Human	Biolegend	Cat: 304017; RRID: AB_389314
NOS3-pSER1177 C9C3 1 in 100 Mouse/Human	Cell Signaling	Cat: 9570S; RRID: AB_823493
NOS3 33/eNOS 1 in 20 Mouse/Human	BD Bioscience	Cat: 560103; RRID: AB_1645506
NOS2 CXNFT 1 in 100 Mouse/Human	eBioscience	Cat: 17-5920-80; RRID: AB_2573243
Bacterial and Virus Strains
pHAGE2-EF1aFull-MCS-IRES-Tomato	Kind gift of G. Mostoslavsky; modified from J Immunol Methods. 2008;329:31–44	N/A
pTWG-CMV-LUC-eGFP	Modified from pTWG-eGFP (Addgene) and N/A pGL4-50 luciferase reporter (Promega)	N/A
Biological Samples
See Tables 1, S1, and S2	N/A	N/A
Chemicals, Peptides, and Recombinant Proteins
Hypoxyprobe Plus Kit	Natural Pharmacia International	HP2-1000Kit
HypoxiSense 680 Fluorescent Imaging Agent	PerkinElmer	NEV11070
DAF-FM DA solution	Sigma-Aldrich	D2321
CellROX Deep Red Reagent	Thermo Fisher Scientific	C10422
NG-Methyl-L-arginine acetate salt (NMMA)	Sigma-Aldrich	M7033
Caveolin-1 scaffolding domain peptide (Cavtratin)	Enzolifesciences	ALX-153-064-M005
S-Nitroso-N-acetyl-DL-penicillamine (SNAP)	Sigma-Aldrich	N3398
Deposited Data
RNA-sequencing data in CD31+ BM-derived endothelial cells	GSE88905	http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE88905
Experimental Models: Cell Lines
HL60	ATCC	Cell Service, The Francis Crick Institute
ML1	ATCC	Cell Service, The Francis Crick Institute
U937	ATCC	Cell Service, The Francis Crick Institute
Experimental Models: Organisms/Strains
NOD-SCID IL2Rgnull (NSG)	Jackson Laboratory	005557
NSG-NESTIN-GFP	The original strain is a kind gift from Dr G. Enikolopov. Back-crossed to NSG background at the BRF, The Francis Crick Institute (generation 8)	N/A
NSG-NG2-DSRED	Original strain from Jackson Laboratory (008241). Back-crossed to NSG background at the BRF, The Francis Crick Institute (generation 8)	N/A
C57BL/6	Jackson Laboratory	000664
C57BL/6 Nos3-KO	Jackson Laboratory	002684
NSG- KitW41/W41 (NBSGW)	Jackson Laboratory	026622
C57BL/6-Rag2-KO-GammaC-KO	BRF, The Francis Crick Institute	N/A
Oligonucleotides
Human VEGFA REV	Sigma-Aldrich	5’-TGGCCTTGGTGAGGTTTGATCC-3’
Human NOS2 FW	Sigma-Aldrich	5’-GGTGCTGTATTTCCTTACGAGGCG-3’
Human NOS2 REV	Sigma-Aldrich	5’-CTTGTTAGGAGGTCAAGTAAAGGGC-3’
Human NOS3 FW	Sigma-Aldrich	5’-CGGCATCACCAGGAAGAAGA-3’
Human NOS3 REV	Sigma-Aldrich	5’-CATGAGCGAGGCGGAGAT-3’
Human GAPDH FW	Sigma-Aldrich	5’-GGGAAGGTGAAGGTCGGAG-3’
Human GAPDH REV	Sigma-Aldrich	5’-GGGTCATTGATGGCAACAA-3’
Murine NOS3 FW	Sigma-Aldrich	5’-CCTTCCGCTACCAGCCAGA-3’
Murine NOS3 REV	Sigma-Aldrich	5’-CAGAGATCTTCACTGCATTGGCTA-3’
Murine NOX4 FW	Sigma-Aldrich	5’-ACTTTTCATTGGGCGTCCTC-3’
Murine NOX4 REV	Sigma-Aldrich	5’-AGAACTGGGTCCACAGCAGA-3’
Murine GAPDH FW	Sigma-Aldrich	5’-AACTTTGGCATTGTGGAAGG-3’
Murine GAPDH REV	Sigma-Aldrich	5’-CACATTGGGGGTAGGAACA-3’
Software and Algorithms
ZEN microscope software	Zeiss	N/A
IMARIS 8.3.1 image analysis software	Bitplane	N/A