Skip to main content
. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Brief Funct Genomics. 2017 Apr 24;16(6):379–389. doi: 10.1093/bfgp/elx007

Figure 2.

Figure 2

Centrifugation and electrophoresis to identify the supercoils present in circular DNA sequences. (A) Sucrose-gradient sedimentation and agarose gel electrophoresis differentiate DNA supercoil level based on the preferential migration of highly supercoiled/writhed molecules. (B) 1D agarose gel electrophoresis in the presence of low concentrations of an intercalator can differentiate topoisomers containing defined numbers of supercoils. (C) Chloroquine gels can be used to differentiate positively and negatively supercoiled DNA. (D) 2D agarose gel electrophoresis differentiates positive and negative supercoil topoisomers. (A colour version of this figure is available online at: https://academic.oup.com/bfg)