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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Brief Funct Genomics. 2017 Apr 24;16(6):379–389. doi: 10.1093/bfgp/elx007

Figure 3.

Figure 3

Psoralen-based approaches identify enrichments of under-wound DNA in eukaryotic genomes in vivo. (A) Approaches taking advantage of inter-strand cross-links formed by a proportion of covalently linked psoralen molecules. Denaturing gel/solution followed by electrophoresis/hydroxyapatite chromatography/exonuclease digestion permits the separation of DNA molecules with an inter-strand cross-link compared with those with no cross-links or a psoralen mono-adduct. Enrichment for inter-strand cross-links at particular loci is then assayed by Southern blot, slot blot, microarray or sequencing. (B) Biotin-psoralen pull-down approaches enrich for psoralen-bound DNA for analysis by microarray or sequencing. (A colour version of this figure is available online at: https://academic.oup.com/bfg)