Figure 2.

The DEPTOR siRNA efficacy and influence of DEPTOR knockdown on the proliferation of OS cells.

Notes: (A) Relative expression of DEPTOR mRNA in MG63 and MNNG/HOS cells after transfection with NC siRNA or DEPTOR siRNA for 24 h. Results are presented as the fold change in DEPTOR siRNA transfected cells relative to that in NC siRNA transfected cells. (B) Western blot analysis of DEPTOR protein when transfected with DEPTOR siRNA or NC siRNA in MG63 and MNNG/HOS cells. (C, D) The proliferation of MG63 (C) and MNNG/HOS (D) cells transfected with NC siRNA or DEPTOR siRNA. Data are expressed as mean ± SD of three independent experiments (*P<0.05, **P<0.01, ***P<0.001 compared with nonspecific siRNA group).

Abbreviations: DEPTOR, DEP-domain containing mTOR-interacting protein; NC siRNA, nonspecific siRNA; OS, osteosarcoma.