Figure 3. Il15ra−/− osteoblasts have defective mineralization and lower rankl/opg ratio.
qPCR analysis of collagen 1a (A), osteocalcin (B) and alkaline phosphatase (C) at 0, 5 and 12 days of osteoblast differentiation (n = 7-5). (D) Alizarin mineralization assay and (E) quantification (n=7-5). (F,G) Rankl/Opg mRNA ratio at 5 (C) and 12 (D) days of differentiation (n = 5).