Figure 2.

Sore throat TLF neutrophils have a distinct activation phenotype. TLF neutrophils were identified by FACS analysis on a SCC^hi CD66b^lo-hi CD16^lo-hi gate and evaluated for (a) ROS production with DHR 123 dye and plasma membrane expression of (b) CD66b, CD16, CD54, and CD11b using appropriate antibodies. Data (left panel) are representative overlaid histogram result from a sore throat and a nonsore throat patient where solid lines represent indicated antibodies and dotted lines show respective isotype antibody controls. Mean results (right panel) are shown as the MFI ± SD derived from sore throat patients (n = 13) and nonsore throat (n = 18) patients. DHR, dihydrorhodamine; MFI: mean fluorescence intensity; TLF: tracheal lavage fluid.