Table 1.
Dog erythrocyte antigen (DEA) 1 blood typing results of anemic patients and healthy dogs assessed by flow cytometry
| Blood typea | Dogs | DEA 1 MFI |
|---|---|---|
| n (%) | Median (range) | |
| Anemic patients | 80 | |
| DEA 1− | 17 (21.3)b | 4 (2–9) |
| DEA 1+ | 63 (78.7)b | 248 (22–686) |
| Weakly DEA 1+ | 18 (28.6)c | 46 (22–74) |
| Moderately DEA 1+ | 22 (34.9)c | 183 (109–287) |
| Strongly DEA 1+ | 23 (36.5)c | 467 (308–686) |
| Healthy dogs | 79 | |
| DEA 1− | 34 (43.0)b | 5 (3–8) |
| DEA 1+ | 45 (57.0)b | 272 (14–954) |
| Weakly DEA 1+ | 9 (20.0)c | 46 (14–70) |
| Moderately DEA 1+ | 19 (42.2)c | 200 (114–283) |
| Strongly DEA 1+ | 17 (37.8)c | 473 (316–954) |
n (%), number (percentage) of dogs, MFI, Mean Fluorescence Intensity.
DEA, Dog erythrocyte antigen. DEA 1 expression was classified as negative (MFI < 10), weakly positive (10 ≤ MFI < 100), moderately positive (100 ≤ MFI < 300), and strongly positive (MFI ≥ 300).
a
Categorical data of DEA 1+ and DEA 1− were completely concordant with immunochromatographic strip technique.
b
Percentage of total dogs.
c
Percentage of DEA 1+ dogs to weak moderate and strong.