Table 2. Substrate specificity of heterologous expressed bile salt hydrolases in Escherichia coli.

	T-β-MCA1	TCA	TCDCA	TDCA	GCA	GCDCA
L. johnsonii LB1 BSHA	-	-	-	-	-	-
L. johnsonii LB1 BSHB	+2	+	+	+	+	+
L. johnsonii LB1 BSHC	+	+	+	+	+	+
L. johnsonii NCK88 BSHB	+	+	+	+	+	+
L. johnsonii NCK88 BSHC	-	-	-	-	+	+
L. johnsonii ATCC 33200 BSHA	-	-	-	-	-	-

¹ Bile acids are: T-β-MCA, tauro-beta-muricholic acid; TCA, taurocholic acid; TCDCA, taurochenodeoxycholic acid; TDCA, taurodeoxycholic acid; GCA, glycocholic acid; GCDCA, glycochenodeoxycholic acid.

² Positive activity is defined as a mean 20% reduction in substrate concentration from three independent assays.