Fig 7. Graphs depicting changes in mRNA expression over time of mx, tnfα, ifnγ, t-bet, and nitr9 in liver, kidney and spleen after treatment with R848.

Only tissues and genes that demonstrated significant changes in expression compared to PBS injected controls are presented. Fold changes in mx in liver (A), kidney (B), and spleen (C), ifnγ in liver (D), kidney (E) and spleen, (F), t-bet in liver (G), and kidney (H) and nitr9 in liver (I) and kidney (J) are presented as mean fold change relative to the time zero group ± standard deviations measured by quantitative RT-PCR. Arp was used as a housekeeping gene. hpi = hours post injection; control = PBS (endotoxin-free); Treated = R848. *Significant (p<0.05) difference in expression of treated compared to control. No significant changes in expression were observed in t-bet and nitr9 in spleen (S1 Table).