Table 1. Oligonucleotide primers and probes used for qRT-PCR to quantify gene expression levels of mx, tnfα, ifnγ, t-bet and nitr9.

Gene	Oligonucleotide sequences (5’–3’)	GenBank Accession No.
arp	Fwd:CTGCAAAGATGCCCAGGGA	NM_131580
	Rev:TTGGAGCCGACATTGTCTGC
	Probe:[6~FAM]TTCTGAAAATCATCCAACTGCTGGATGACTACC [BHQ1a~ Q] [47]
mx	Fwd:GCATCATTAGTTCAGACAGTCG	NM_182942.4
	Rev:AAATTATCGATAGTGTCGATACAAG
	Probe:[6~FAM]TGCTGACTGAACGTGTAACTCAACT [BHQ1a~ Q] *
tnfα	Fwd:TCGCATTTCACAAGGCAATTT	NM_212859
	Rev:GGCCTGGTCCTGGTCATCTC
	Probe:[6~FAM]AGGCTGCCATCCATTTAACAGG[BHQ1a~Q] [47]
ifnγ	Fwd:CTTTCCAGGCAAGAGTGCAGA	NM_212864
	Rev:TCAGCTCAAACAAAGCCTTTCG
	Probe:[6~FAM]AACGCTATGGGCGATCAAGGAAAACGAC[BHQ1a~ Q] [47]
t-bet	Fwd:GATCAAGCTCTCTCTGTGATAG	NM_001170599.1
	Rev:GCTAAAGTCACACAGGTCT
	Probe:[6~FAM]TTCTGAAGGTCACGGTCACA[BHQ1a~Q] *
nitr9	Fwd:GTCAAAGGGACAAGGCTGATAGTT	AY570237.1
	Rev:GTTCAAAACAGTGCATGTAAGACTCA
	Probe: [6~FAM]CAAGGTTTGGAAAAGCAC[BHQ1a~Q] [48]

Housekeeping gene arp (house-keeping gene) was used as a reference gene.

* The mx and t-bet primers and probes were designed by Beacon Design software (BioRad) and Primer3 plus (GraphPad) software, respectively.