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. 2017 Sep 14;12(9):e0184461. doi: 10.1371/journal.pone.0184461

Fig 6. Fyn and Src target to the apical membrane surface during EC lumen formation.

Fig 6

(A) ECs were allowed to form lumens and after 24 hr, were immunostained with antibodies specific for Src, Yes or Fyn. ECs were counterstained with phalloidin to label F-actin and Hoescht to label nuclei. Arrowheads indicate apical staining of Fyn or Src while arrowheads indicate the basal staining of F-actin. (B) ECs with lumens were immunostained with the indicated phospho-antibodies. Arrowheads indicate apical staining of phospho-Src (Y416) and phospho-c-Raf (Y314). L indicates an EC luminal space. Bar equals 25 μm.