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. 2017 Sep 14;12(9):e0184644. doi: 10.1371/journal.pone.0184644

Fig 1. Expression patterns of LIN28B and the let7-microRNA (miRNA) family in human castration-resistant prostate cancer (CRPC) cells.

Fig 1

A. Comparison of LIN28B expression levels in human CRPC cells (PC3) compared to those in the normal prostate cell line (RWPE1) by (a) quantitative reverse transcriptase-polymerase chain reaction (qPCR) for LIN28B mRNA expression and (b) western blot analysis for protein expression. In qPCR, relative mRNA expression is normalized to GAPDH expression levels, and finally presented in fold change as mean ± S.E.M (n = 3). In western blot analysis to determine the relative expression of LIN28B protein, beta-actin was used as the loading control. B. Comparison of let7-miRNA expression between RWPE1 and PC3 cell lines by qPCR analysis. Relative miRNA expression is normalized to U6 expression levels, and finally presented in fold change as the mean ± S.E.M (n = 3).