Table 1. Microsatellite loci for L. thermotolerans genotyping.

Locus	Chr.	Coordinates	Motif	Primersb	Dye	Tm	Number of alleles	Size range	Coding sequence	Function
LT2A	2	610672–610712	ACA	F:TGACAAAAGTTTATCCCCCC	NED	62	24	385–438	XP_002552115	RNA-binding protein
				R:AGCACTGGCGATATCTTGGTT
LT3A	3	129153–129184	AGC	F:CAGTACCAGCGCCAGTTCTA	PET	60c	25	293–352	XP_002552291	peroxin family member
				R:TTCTGTAGCTTGGGGTTGTGT
LT3B	3	621739–621768	AGC	F:ACAGCAGCAGCAACAGCAA	NED	60c	9	86–111	no similarity found	na
				R:TTCGCCAAGCTGCTGATACTA
LT4A	4	897528–897557	AGA	F:AGAAGGAGGACTCAGCGGATT	NED	60c	12	222–260	no similarity found	na
				R:ATGCCTAAGCGAATCAGATGC
LT5B	5	317191–317225	ATA	F:AACGCTGACGTGCTGAAAGA	FAM	56	10	275–314	no similarity found	na
				R:GAAAGAGGCAGTAACGGATTT
LT6B	6	134618–134640	ACA	F:TTCCTAGGTCTGGACCTCCAA	PET	60c	24	106–161	no similarity found	na
				R:TATTGCTGCTGCTTTTGCTG
LT7A	7	1417616–1417644	TGT	F:TTTTTTCTTGATGCCCCGGT	FAM	60	10	131–150	XP_002555739	unknown; kinase suppression effect
				R: CGAACTGTGGTTCCTTCACAT
LT8A	8	638186–638223	TCC	F:TGAAATAGAGTCCCGTGTGAA	PET	62	28	182–240	XP_002556192	vacuolar protein sorting
				R: AAATAACGCAGAAAGCGAGG
LT8B	8	239222–239256	ATG	F:CAGCATCCGCACAGTAGCTAA	HEX	60c	9	261–286	XP_002555998	nuclear DNA helicase
				R:TTATCTCCTTATGCGGGCGTA
MA2a	1	358081–358339	CA	F:AATTTTACGAAGGGAGAGAGGG	NED	60c	44	298–358	XP_002551596	bud-site selection nutrient signaling
				R:CTGCTGATGGTTTCTTCTGTGA
MD3a	4	259537–259789	CAA	F:ACAAGAAAGCGAAGGAAAACAG	FAM	62	41	353–485	XP_002552792	unknown; hypothetical ORF
				R: CCCAGTAGAACGTGATTAAGCC
ME11a	5	1381401–1381503	TG	F:CGGTTCTTAGCTTACCAACAGC	HEX	52	30	148–209	XP_002554109	mitotic spindle-associated protein
				R:ACTCGAACAGCCAGAGCTTAAC
ME4a	5	576050–576253	GA	F:TGGCCTCTTCTGTCTTTCCTAA	HEX	60c	34	346–421	no similarity found	na
				R:CTCATCAACCAACACACTCCAT
MH6a	8	372940–373089	TGT	F:CTTGCTGTTGTCGTAACCTCTG	PET	62	49	374–566	XP_002556014	ER-associated protein degradation; hypothetical ORF
				R:AATCCCAATAATCTCACACCC

Chr.—chromosome; Tm—melting temperature.

^a Banilas et al. (2016)

^b M13 sequence was attached at the 5’ end of the forward primer

c touch-down PCR commenced at Tm + 10°C with a 1°C decrease per cycle (see Materials and Methods).