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. 2017 May 26;6(9):e1333213. doi: 10.1080/2162402X.2017.1333213

Figure 2.

Figure 2.

AIF1 was associated with the proliferation, migration, and M2 polarization of macrophages. (A) Inhibiting AIF1 with siRNA transfection decreased the number of RAW264.7 cells. (B) Phalloidin-TRITC staining showed that inhibiting AIF1 with siRNA transfection resulted in malformation of the cytoskeleton. (C, D) RAW264.7 cells were less attractant to CSF1 stimulation (100 ng/mL) after constitutive knockdown of AIF1 and more attractant to CSF1 stimulation (100 ng/mL) after constitutive overexpression of AIF1. (E, F) Western blot confirmed AIF1 was effectively knocked down or overexpressed after infection with lentivirus containing shRNA targeting AIF1 or transfection with the AIF1 overexpression plasmid. (G) QRT-PCR showed that overexpression of AIF1 in RAW264.7 cells exhibited increased markers associated with M2 polarization and decreased those associated with M1 polarization. (H) Flow cytometric assay showed that overexpression of AIF1 in RAW264.7 cells showed increased CD206 and decreased MHC-II; *p < 0.05; ** p < 0.01; ***p < 0.001.