Skip to main content
. 2017 May 26;6(9):e1333213. doi: 10.1080/2162402X.2017.1333213

Figure 4.

Figure 4.

AIF1 enhanced the proliferation and migration of Hepa1-6 by the secretion of CXCL16. (A) In the Corning 3422 Boyden chambers, RAW264.7 cells in the lower chamber were co-cultured with Hepa1-6 cells in the upper chamber for 24 h. The migration of Hepa1-6 cells was attenuated with RAW264.7 cells downregulating AIF1 and enhanced with RAW264.7 cells overexpressing AIF1. (B) The conditioned medium of RAW264.7 cells downregulating AIF1 decreased the number of Hepa1-6 cells, and the conditioned medium of RAW264.7 cells overexpressing AIF1 increased the number of Hepa1-6 cells. (C) AIF1 promoted the migration of Hepa1-6 cells, which was blocked by the neutralizing antibody against CXCL16; *p < 0.05; **p < 0.01; ***p < 0.001.