Figure 4.

CD4⁺ systemic immune response measured in the peripheral blood. (A) Following vaccination, responsivity increased significantly in each group (ER^neg 45.8% → 91.7%; ER^pos w/o AE 50.0% →75.0%; ER^pos w AE 57.1% →90.5%, all p < 0.01). Pre-vaccination responsivity rates were similar across all three groups (ER^neg 45.8%, ER^pos w/o AE 50.0%, ER^pos w AE 57.1%; p = 0.75). Post-vaccination responsivity rates were also similar across all three groups (ER^neg 91.7%, ER^pos w/o AE 75.0%, ER^pos w AE 90.5%; p = 0.41). (B) Following vaccination, median response repertoire increased in each group (ER^neg 0 → 3, p = 0.03; ER^pos w/o AE 0.5 →1.5, p = 0.08; ER^pos w AE 1 →2, p = 0.03). Pre-vaccination median response repertoires were similar across all three groups (ER^neg 0 (IQR 0–2), ER^pos w/o AE 0.5 (IQR 0–1.5), ER^pos w AE 1 (IQR 0–1); p = 0.99). Post-vaccination median response repertoires were also similar across all three groups (ER^neg 3 (IQR 1–4), ER^pos w/o AE 1.5 (IQR 0.5–4), ER^pos w AE 3 (IQR 2–4); p = 0.67). (C) Following vaccination median cumulative response increased in each group (ER^neg 47.6 →143.8; ER^pos w/o AE 41.1 →178.7; ER^pos w AE 58.6 →100.9, all p < 0.01). Pre-vaccination median cumulative responses were similar across all three groups (ER^neg 47.6 (IQR 23.3–102.6), ER^pos w/o AE 41.1 (IQR 9.6–168.6), ER^pos w AE 58.6 (IQR 24.2–87.9); p = 0.94). Post-vaccination median cumulative responses were also similar across all three groups (ER^neg 143.8 (IQR 97.8–238.3), ER^pos w/o AE 178.2 (IQR 64.7–278.3), ER^pos w AE 100.9 (IQR 67.5–174.4); p = 0.62).