Figure 5.
Cross-reactivity of the immunological responses elicited by the mutated analogs of SIINFEKL. Splenocytes from mice treated with SIINFEKL, SIINFAKL or SIIWFEKL (presented in Fig. 4, here indicated on the x axis), were assayed for cross-responses to the peptides. 3 × 105 cells were incubated with 100 ng of each indicated peptide for 72 h and the IFNγ response was determined by ELISPOT assay. (A) The number of Spot-Forming Units (SFU) is normalized to 1 × 106 cells. The red-dotted line represents the background of the assay (splenocytes incubated with PBS). (B) The cumulative size of the spots from mice of the same group (same column) in response to different stimuli (bars of different colors) is shown. Data is shown as mean ± SEM. C) The average spot size across all the groups in response to each peptide is represented. Data is shown as the mean ± SEM; One-way ANOVA with Tukey's post hoc test was used to calculate the statistical significance.