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. 2017 Aug 10;6(9):e1342024. doi: 10.1080/2162402X.2017.1342024

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Figure 2. — BM and PB CD56^lowCD16^low NK-cell phenotype at different time points after transplant. Cells freshly isolated by density gradient centrifugation from BM and PB of pediatric healthy donors and haplo-HSCT recipients were analyzed by flow cytometry for the expression of activating receptors (panel A), MHC class I-specific inhibitory receptors (panel B), differentiation markers (panel C) and cytokines receptors (panel D). The CD56^highCD16^+/− (gray), CD56^lowCD16^low (white) and CD56^lowCD16^high (black) NK cell subsets were gated on CD56⁺CD3⁻ NK cells. Histograms represent the mean value ± SD of the percentage of positive cells analyzed at different time-points after HSCT starting from the first month until 12^th month.