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. Author manuscript; available in PMC: 2017 Nov 23.
Published in final edited form as: J Am Chem Soc. 2017 Aug 10;139(33):11337–11340. doi: 10.1021/jacs.7b06555

Figure 2.

Figure 2

Small molecule induced acetylation of H3K27 at IL1RN locus. (a) Constructs of dCas9-PYL-HA and FLAG-ABI-P300. (b) The sites targeted by sgRNAs (red stars) and locations probed by qPCR amplicon (black solid circles) at the IL1RN locus. (c) Enrichment of dCas9-PYL-HA at the IL1RN locus with or without sgRNAs. Enrichment of FLAG-ABI-P300 (d) and H3K27Ac (e) at the IL1RN locus with or without ABA treatment. (f) Levels of IL1RN mRNA. ** represents P<0.01 compared with control group. Fold changes were calculated by comparing to cells treated without sgRNA (c), with DMSO (d, e) or transfected with dCas9-PYL (f). Error bars represent ± s.e.m. from biological replicates (n = 5).