Skip to main content
. Author manuscript; available in PMC: 2018 Sep 5.
Published in final edited form as: Structure. 2017 Jul 27;25(9):1337–1347.e6. doi: 10.1016/j.str.2017.06.018

Figure 1. Initial characterization of ANK17 binding to Snap25b.

Figure 1

(A) Schematic topology of DHHC17 palmitoyl-transferase. (B) Two-step reaction scheme for a typical DHHC enzyme. (C) List of constructs used for crystallization: ANK17 (corresponding to N-terminal domain of Human DHHC17 - residues 51–288) and full-length Snap25b-WT were produced by recombinant expression in E.coli. Peptide corresponding to amino acids 111 to 120 in Snap25 was produced by solid phase peptide synthesis (D) Representative ITC thermograms and non-linear regression analyses for the interaction between ANK17 and Snap25b (left panel), Snap25b-C4A (central panel) or S25b111–120 (right panel).