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A) ANK17-WT or ANK17-mutants titrated into Snap25b. ANK17-N100A and ANK17-W130A showed only heat of dilution. (B) ANK17-WT titrated into Snap25b mutants. Snap25b-P117A showed only marginal heat of binding and we could not obtain any fitting of the integrated heats. (C) Position of mutated residues in ANK17 and Snap25b in the structure of the complex. (D) Histogram representing binding affinity (Kd) determined by ITC titrations (n.d.: not determined).
