Table 1.
EC50 of dapivirine against a panel of influenza A and B viruses and selection index of dapivirine with MDCK and A549 cell lines.
| Influenza Strains | Drug sensitivity | EC50 (μM)a | SIc |
|---|---|---|---|
| A/DM/528/2009 (H1N1) | Amantadine Resistant Oseltamivir Resistant |
1.3 ± 0.2 | 11.0 |
| A/Texas/4/2009 (H1N1) | 1.8 ± 0.3 | 7.9 | |
| A/North Carolina/39/2009 (H1N1) | 1.1 ± 0.1 | 13.0 | |
| A/Washington/29/2009 (H1N1) | 1.2 ± 0.2 | 11.9 | |
|
| |||
| A/WSN/1933 (H1N1) | Amantadine Resistant Oseltamivir Sensitive |
1.8 ± 0.2 | 7.9 |
| A/California/07/2009 (H1N1) | 1.2 ± 0.1 | 11.9 | |
| A/DM/524/2009 (H1N1) | 1.4 ± 0.1 | 10.2 | |
| A/Switzerland/9715293/2013 (H3N2) | 1.4 ± 0.2 | 10.2 | |
| A/Switzerland/9715293/2013 X-247 (H3N2) | 1.1 ± 0.3 | 13.0 | |
| A/Hongkong/4801/2014 (H3N2) | 1.4 ± 0.2b | 10.2 | |
| A/California/2/2014 (H3N2) | 2.2 ± 0.3 | 6.5 | |
|
| |||
| A/Udorn/1972 (H3N2) | Amantadine Sensitive Oseltamivir Sensitive |
1.3 ± 0.4 | 11.0 |
| A/Soloman Islands/3/2006 (H1N1) | 1.5 ± 0.2 | 9.5 | |
|
| |||
| B/Wisconsin/1/2010 (Yamagata) | Amantadine Resistant Oseltamivir Sensitive |
1.2 ± 0.3 | 11.9 |
| B/Bris/60/2008 (Victoria) | 1.1 ± 0.1 | 13.0 | |
a
All EC50 results were determined in plaque assays using the ST6Gal I–overexpressing MDCK cells (AX-4) in 6-well plates; The value is the mean of two independent experiments ± standard deviation.
b
EC50 was determined by CPE assay; The value is the mean of two independent experiments ± standard deviation.
c
SI refers to selectivity index, which is calculated by dividing the 48 hrs CC50 of dapivirine using MDCK cells by the EC50 of dapivirine.