Figure 2.

Global Transcriptome Profiling by RNA-Seq Reveals Differences between WT and HEB^−/− hESCs

(A) Heatmap showing differential gene expression patterns of three replicates each for WT and HEB^−/− hESCs (clone KO-4).

(B–D) Expression of E proteins (B), genes associated with pluripotency (C), and TGFβ family members (D) in undifferentiated WT and HEB^−/− hESCs, as determined by RNA-seq. FPKM, fragments per kilobase of exon per million fragments mapped. Error bars represent mean ± SD (n = 3 biological replicates). ^∗∗p < 0.01 by Student's t test. See also Figures S2 and S3; Table S2.