Figure 7.

HEBCan Rescues Hematopoiesis and T Cell Development in HEB^−/− hESCs in OP9-DL4 Co-cultures

(A) qRT-PCR analysis for the expression of hematopoietic genes in CD34⁺ cells sorted from WT, KO + GFP, and KO + HEBCan day-8 (d8) EBs. mRNA levels are shown relative to GAPDH.

(B and C) Percentages (B) and numbers (C) of CD45⁺ cells in d12 and d18 OP9-DL4 co-cultures.

(D and E) Flow-cytometric analysis of T cell development from WT, KO + GFP, and KO + HEBCan d8 EB-derived CD34⁺ cells at d12 (D) and d18 (E) of OP9-DL4 co-culture. Cells are gated on the CD45⁺DAPI⁻ population.

Error bars represent mean ± SD (n = 3 independent experiments). ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005 by Student's t test. Plots in (B), (D), and (E) are representative of three independent experiments.