Table 1.

Analytical methodologies used to compare Remicade and Remsima.

Analytical Method	Sample Processing	Measured Attributes	Method Advantages
Native MS, IM-MS	Buffer exchange, Deglycosylation, IdeS digest	Intact mass, fragments, aggregation, glycation, higher order structure, gas-phase stability	Limited sample preparation, rapid analysis, low detection limit and high information content
Denaturing MS	Deglycosylation and reduction	Intact masses of subunits, C-terminal Lys truncation	Information on accurate subunit mass and heterogeneity
Peptide Mapping	Trypsin digest,Glu-C digest	Sequence, mutations, oxidations, deamidations, glycosylation Glycations	Single analysis allows for identification and quantification of multiple attributes
Size Exclusion Chromatography	None	Level of Aggregation	N.A.
Biolayer Interferometry	Buffer exchange	Fc receptor binding KD	N.A.

N. A. - Not applicable.