Figure 1.

Biphasic Changes in Neurogenesis after Brain Injury

(A–D) Images show doublecortin (DCX) immunostaining in sections from rats sacrificed 3 days (A, sham; B, FPI) and 30 days (C, sham; D, FPI) after moderate FPI or sham injury.

(E) Quantification of DCX-labeled cells in sections from rats sacrificed 3, 7, 30, and 90 days after FPI are compared with data from age-matched sham-injured controls. Main effect of injury on DCX cell counts F_{(1, 26)} = 1.378; p = 0.251. Main effect of post-injury time on DCX cell counts F_{(3, 26)} = 26.576; p < 0.001. Interaction between injury and time was significant (F_{(3, 26)} = 10.514; p < 0.001).^∗p < 0.05 compared with sham and ^#p < 0.05 compared with 3-day sham by two-way ANOVA (TW-ANOVA) and pairwise post hoc comparisons. n = 4 sham and 5 FPI rats/group. Pairwise comparisons between all FPI groups were significant.

(F) Example of the contour tracing used to outline the dentate subgranular zone for stereological cell counts.

d, day; GLC, granule cell layer; SGZ, subgranular zone. Data are presented as means ± SEM.