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. 2017 Aug 17;9(3):927–942. doi: 10.1016/j.stemcr.2017.07.013

Figure 3.

Figure 3

Inhibition of ERK/MAPK Signaling or EMT Rescues the Differentiation Phenotype Caused by Jmjd1c Depletion

(A) Bright-field images of Jmjd1c-depleted and control ESCs after treatment with DMSO or the MEK1/2 inhibitor PD0325901 (1 μM) for 4 days.

(B and C) qRT-PCR analysis of transcript levels for pluripotency (B) and lineage (C) genes in Jmjd1c-depleted and control ESCs after treatment with DMSO or the MEK1/2 inhibitor PD0325901 (1 μM) for 4 days. Data are presented as mean ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, not significant.

(D and E) Expression levels of EMT markers in Jmjd1c-depleted and control ESCs after treatment with DMSO or the MEK1/2 inhibitor PD0325901 (1 μM) for 4 days. Data are presented as mean ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, not significant.

(F) Bright-field images of Jmjd1c-depleted and control ESCs after treatment with DMSO or the MMPs inhibitor GM6001 (50 μM) for 4 days.

(G and H) Expression levels for EMT markers in Jmjd1c-depleted and control ESCs after treatment with DMSO or the MMPs inhibitor GM6001 (50 μM) for 4 days. Data are presented as mean ± SD of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ns, not significant.