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. 2017 Aug 10;9(3):807–819. doi: 10.1016/j.stemcr.2017.07.012

Figure 3.

Figure 3

Effect of Nutrient Supplementation on Islet Cell Death In Vitro

(A) SCIPC and mouse islets were cultured at various densities in complete RPMI with or without addition of glucose. Cell viability was quantified using PI staining via flow cytometry. Data are a compilation of results from three independent experiments (SCIPC: n = 9 per group; mouse: n = 12 per group).

(B) Mouse islet survival in high-density cultures supplemented with selected amino acids. Ala, alanine; Gln, glutamine; Cys, cysteine; Trp, tryptophan; Leu, leucine; Met, methionine. Data are a compilation of results from three independent experiments (n = 9 per condition).

(C) SCIPC and human islet survival in high-density cultures (SCIPC: 3,000 clusters/mL; human islets: 1,000 islets/mL) supplemented with selected amino acids. Cell viability was quantified as described in (A). Data are a compilation of results from three independent experiments (n = 9 per condition).

For all panels, statistical significance of differences from the high-density condition of the same cell type was determined using one-way ANOVA with Holm-Sidak multiple comparisons. All data are expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant.