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. 2017 Aug 30;6:e25301. doi: 10.7554/eLife.25301

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© 2017, Wells et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — Graph Legend: Red is the percentage of R8 photoreceptors that expressed Rh6, Blue is the percentage that expressed Rh5 and Purple is Co-expression of Rh5 + Rh6. Error bars report standard error of the mean. Paired t-tests were performed against percentages of Rh5 expression and significance is denoted where applicable (***=p≤0.01, **=p≤0.05, *=p≤0.1, ns = not significant). Where significance (or non-significance) is noted, follow the horizontal bar above the significance value to its termination in a vertical tick to find the comparative genotype. Percentage values for each genotype are listed as (Rh5/(Rh5 + Rh6)/Rh6). (A) Expressing UAS-Babo* rescued some yellow subtypes induced by removing dActβ (lGMR-Gal4 > UAS-Dcr2; UAS-Babo*; UAS-dActβRNAi) (57/0/43) (n = 6), daw (to a lesser extent) (lGMR-Gal4 > UAS-Dcr2; UAS-Babo*; UAS-DawRNAi) (21/1/78) (n = 23) (B) and myo (lGMR-Gal4 >UAS-Dcr2; UAS-Babo*; UAS-MyoRNAi) (49/2/48) (n = 6) (C). (D) Quantification of A-C. (E) Expressing UAS-Tkv* could not rescue yellow subtypes induced by removing dActβ (lGMR-Gal4 > UAS-Dcr2; UAS-Tkv*; UAS-dActβRNAi) (12/1/86) (n = 6). (F) Tkv* did slightly, yet significantly, rescue loss of daw (lGMR-Gal4 > UAS-Dcr2; UAS-Tkv*; UAS-DawRNAi) (9/18/72) (n = 13) but could not rescue loss of myo (lGMR-Gal4 > UAS-Dcr2; UAS-Tkv*; UAS-MyoRNAi) (14/10/76) (n = 8) (G). (H) Quantification of E-G. (I) Expressing UAS-Tkv* rescued many yellow subtypes induced by removing dpp (lGMR-Gal4 > UAS-Dcr2; UAS-Tkv*; UAS-DppRNAi) (25/18/56) (n = 15) or gbb (lGMR-Gal4 > UAS-Dcr2; UAS-Tkv*; UAS-GbbRNAi) (38/8/54) (n = 12) (J). (K) Expressing UAS-Babo* also rescued many yellow subtypes induced by removing dpp (lGMR-Gal4 > UAS-Dcr2; UAS-Babo*; UAS-DppRNAi) (46/0/54) (n = 7) or gbb (lGMR-Gal4 > UAS-Dcr2; UAS-Babo*; UAS-GbbRNAi) (38/0/62) (n = 6) (L). (M) Schematic for hypothetical ligand dimerization possibilities upstream of Tkv and Babo activation. Dpp + Gbb may heterodimerize to activate Tkv while Daw + dActβ and Daw + Myo may heterodimerize and signal at highly regulated levels for Babo activation. (N) Quantification of I-L. (O) Co-expression of dActβRNAi and MyoRNAi (lGMR-Gal4 > UAS-Dcr2; UAS-dActβRNAi; UAS-MyoRNAi) (1/0/99) (n = 6) exacerbated specification of yellow subtypes induced in dActβRNAi or MyoRNAi retinas alone. However, combining DawRNAi with either dActβRNAi (lGMR-Gal4 > UAS-Dcr2; UAS-dActβRNAi; UAS-DawRNAi) (15/0/85) (n = 7) or MyoRNAi (lGMR-Gal4 > UAS-Dcr2; UAS-MyoRNAi; UAS-DawRNAi) (15/0/85) (n = 12) could not exaggerate the phenotypes of any of the three alone. † denotes a change in Rh5/Rh6 ratio that was significant but in the unexpected direction.