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. 2017 Aug 31;9(3):853–867. doi: 10.1016/j.stemcr.2017.07.020

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Cytotoxic Responses against Regenerated C1/C1 T Cells Is Limited to the KIR2DL1⁺ NK Cell Subsets Isolated from a C1/C2 Donor

(A) Flow-cytometric profiles of KIR2DL1 and KIR2DL3 in NK cells isolated from donor Hetero-1 after sorting by magnetic beads. KIR2DL1⁻ cells (R1/R4) were enriched by negative selection (depletion of KIR2DL1⁺ cells), whereas KIR2DL1⁺ (R2/R3) cells were enriched by positive selection.

(B and C) Six-hour cytotoxicity assays of NK cells isolated from donor Hetero-1 against Homo-A iPSC-TCs. Results are presented as mean ± SD from three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, Student's t test.