Table 1.
Primers used for sequencing
Marker | Primer name | Primer | Reference |
---|---|---|---|
ORF-PolB | AM_ORF314-F1 | AGCGCTATGATTAGAGTATTTAAGG | This study |
AM_ORF314-R1 | TCAATTCTAGTTTAGAGCTTCCTC | This study | |
AM_polB-F1 | ATCCTGTACTAAGCCAAATCATC | This study | |
AM_polB-R1 | ATATAATCGGTCGTTAGTCGGC | This study | |
COI | med-cox1-F | ACNAAYCAYAAAGATATHGG | This study |
med-cox1-R | TGGTGNGCYCANACNATRAANCC | This study | |
16S | med-rnl-F | GACTGTTTACCAAAGACATAGC | This study |
med-rnl-R | AAGATAGAAACCTTCCTGTC | This study | |
ITS | C2 | GAAAAGAACTTTGRARAGAGAGT | Chombard et al., 1997 |
D2 | TCCGTGTTTCAAGACGGG | Chombard et al., 1997 | |
28S | 28S-F63 | AATAAGCGGAGGAAAAGAAAC | Medina et al., 2001 |
28S-R635 | GGTCCGTGTTTCAAGACGG | Medina et al., 2001 |
ORF-PolB primers were designed based on Kayal et al. (2012). COI and 16S were designed based on conserved regions among medusozoan cnidarians that overlap well with the commonly used Folmer et al. (1994) and Palumbi (1996) fragments, respectively.