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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Biol Bull. 2016 Oct;231(2):152–169. doi: 10.1086/690095

Table 1.

Primers used for sequencing

Marker Primer name Primer Reference
ORF-PolB AM_ORF314-F1 AGCGCTATGATTAGAGTATTTAAGG This study
AM_ORF314-R1 TCAATTCTAGTTTAGAGCTTCCTC This study
AM_polB-F1 ATCCTGTACTAAGCCAAATCATC This study
AM_polB-R1 ATATAATCGGTCGTTAGTCGGC This study
COI med-cox1-F ACNAAYCAYAAAGATATHGG This study
med-cox1-R TGGTGNGCYCANACNATRAANCC This study
16S med-rnl-F GACTGTTTACCAAAGACATAGC This study
med-rnl-R AAGATAGAAACCTTCCTGTC This study
ITS C2 GAAAAGAACTTTGRARAGAGAGT Chombard et al., 1997
D2 TCCGTGTTTCAAGACGGG Chombard et al., 1997
28S 28S-F63 AATAAGCGGAGGAAAAGAAAC Medina et al., 2001
28S-R635 GGTCCGTGTTTCAAGACGG Medina et al., 2001

ORF-PolB primers were designed based on Kayal et al. (2012). COI and 16S were designed based on conserved regions among medusozoan cnidarians that overlap well with the commonly used Folmer et al. (1994) and Palumbi (1996) fragments, respectively.

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