. Author manuscript; available in PMC: 2017 Sep 15.

Published in final edited form as: Biol Bull. 2016 Oct;231(2):152–169. doi: 10.1086/690095

Table 1.

Primers used for sequencing

Marker	Primer name	Primer	Reference
ORF-PolB	AM_ORF314-F1	AGCGCTATGATTAGAGTATTTAAGG	This study
	AM_ORF314-R1	TCAATTCTAGTTTAGAGCTTCCTC	This study
	AM_polB-F1	ATCCTGTACTAAGCCAAATCATC	This study
	AM_polB-R1	ATATAATCGGTCGTTAGTCGGC	This study
COI	med-cox1-F	ACNAAYCAYAAAGATATHGG	This study
COI	med-cox1-R	TGGTGNGCYCANACNATRAANCC	This study
16S	med-rnl-F	GACTGTTTACCAAAGACATAGC	This study
16S	med-rnl-R	AAGATAGAAACCTTCCTGTC	This study
ITS	C2	GAAAAGAACTTTGRARAGAGAGT	Chombard et al., 1997
ITS	D2	TCCGTGTTTCAAGACGGG	Chombard et al., 1997
28S	28S-F63	AATAAGCGGAGGAAAAGAAAC	Medina et al., 2001
28S	28S-R635	GGTCCGTGTTTCAAGACGG	Medina et al., 2001

ORF-PolB primers were designed based on Kayal et al. (2012). COI and 16S were designed based on conserved regions among medusozoan cnidarians that overlap well with the commonly used Folmer et al. (1994) and Palumbi (1996) fragments, respectively.