Table 4.
AMOVA and Isolation by Distance (IBD) analyses
| ORF-PolB | COI | 16S | ITS | 28S | |
|---|---|---|---|---|---|
| All four sampled localities | |||||
| Among population variation | 14% | 17% | 9% | 9% | 3% |
| Within population variation | 86% | 83% | 91% | 91% | 97% |
| ΦST/FST | 0.137 | 0.170 | 0.086 | 0.088 | 0.032 |
| Atlantic vs. Pacific | |||||
| Among group variation | 31% | 35% | 27% | 4% | 12% |
| Within group variation | −1% | 0% | −2% | 7% | −2% |
| Within population variation | 70% | 65% | 75% | 89% | 90% |
| ΦST/FST | 0.302 | 0.354 | 0.249 | 0.110 | 0.099 |
| ΦSC/FSC | −0.010 | 0.001 | −0.031 | 0.075 | −0.027 |
| ΦCT/FCT | 0.309 | 0.353 | 0.272 | 0.038 | 0.122 |
| Isolation by distance (r) | 0.791 | 0.968 | 0.850 | 0.260 | 0.955 |
| P-value | 0.208 | 0.041 | 0.129 | 0.417 | 0.121 |
Analysis of molecular variance (AMOVA) is represented in the first part of the table with only one group encompassing the entire dataset including all four populations; then with two groups divided by ocean basin (i.e., Pacific containing Saipan, Australia, and Hawaii vs. Atlantic containing Bonaire). The mitochondrial AMOVA was calculated assuming the TrN model of nucleotide evolution. Molecular variance is divided into components of among groups (ΦCT and FCT), among localities within groups (ΦSC and FSC), and among all localities (ΦST and FST). Values in bold are significant (P < 0.05).