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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Pharmacogenomics J. 2017 Mar 14;18(1):136–143. doi: 10.1038/tpj.2016.92

Table 2.

Nicotine N-oxidation activity in human and mouse microsomes and recombinant human enzymes

cis-nicotine-N-oxide (pmol min−1 mg−1)a trans-nicotine-N-oxide (pmol min−1 mg−1)a trans : cis
Human brainb 0.23 ± 0.01 0.22 ± 0.03 0.95
Human astrocytesc 0.30 ± 0.11 1.25 ± 0.42 4.2
Human liverd 271 ± 30 10 400 ± 300 38.4
Mousee brainf 27.6 ± 0.8 1.1 ± 0.2 0.04
Mousee liverd 2 670 ± 150 1 090 ± 40 0.4
FMO3 supersomesg 4 160 ± 1 090 473 000 ± 52 000 114
FMO1 supersomesg 94 600 ± 4 540 4 280 ± 250 0.05
a

5 mM nicotine

b

1.5 mg/ml protein in incubation

c

1.0 mg/ml protein in incubation

d

30 μg/ml protein in incubation

e

pooled tissues from adult female CD1 mice

f

260 μg/ml protein in incubation

g

0.5 μg/ml protein in incubation