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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Hypertension. 2017 Aug 21;70(4):813–821. doi: 10.1161/HYPERTENSIONAHA.117.09456

Figure 4.

Figure 4

Western blots of pS6S235/236 and pAKTS473 were normalized with total endogenous S6 and AKT proteins, respectively. Graphs represent pS6S235/236/S6 or pAKTS473/AKT normalized to the vehicle treated SS rats. Corresponding representative immunoblots are shown below each graph. A, The level of pS6S235/236/S6 and B, Representative photomicrograph of the immunohistochemical profile of pS6S235/236 level in the renal cortex and medulla of SS rats treated with vehicle (n=7) or rapamycin (n=9) fed a 4.0% NaCl diet. White discontinuous lines designate the boundaries of the cortex (C) and medulla (M) C, pAKTS473/AKT in the cortex and outer medulla was measured in these rats kidney. * p<0.05 Vs vehicle.