Fig. 3.

Expression of LDL receptors in cardiomyocytes (CMC) or liver. a Cycle number for the threshold of LDL receptor amplifications of samples from cardiomyocytes cultured for 24 h under control conditions or liver samples (n = 10, 8 independent experiments). Data are normalized to the mean expression of three house-keeping genes (HPRT, GAPDH, B2M). b Relative expression normalized to the expression of the strongest expressed receptor in each tissue. All data are normalized to the three house-keeping genes as an internal standard. c Effect of silencing of LOX-1 by supplying siRNA direct against LOX-1. oxLDL concentration was 20 µg/ml. ScrambleRNA represents a randomized sequence used as a control. Data are mean ± SD from 72 cells (nine independent experiments). a: p < 0.05 vs. control, siRNA, siRNA + oxLDL, and scrambleRNA