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. 2017 Sep 14;112(6):63. doi: 10.1007/s00395-017-0650-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Expression of PCSK9 in liver and cardiomyocytes (CMC). a Cycle number for the threshold of PCSK9 amplification of the samples are given for (n = 10, eight independent experiments, CMC) and liver isolated from normotensive rats (n = 5). Data are normalized to the mean expression of the three house-keeping genes GAPDH, HPRT, and B2M. b Relative expression of PCSK9 in cardiomyocytes normalized to the expression in liver. All data are normalized to the three house-keeping genes. Data are mean ± SD. p > 0.05. c Representative immunoblot indicating the expression of PCSK9 (top) and GAPDH (bottom). Original blot is shown in supplementary Figure 1. d Release of PCSK9 into the medium (PM plain culture medium, CM conditioning medium, 24 h incubation, n = 6); data shown are mean ± SD from n independent preparations