Table 4.
ZIKV/DENV antibody discrimination in the four main computational steps
| Per serum, without and with () urea, MFI-cutoff > 100 | ZIKV IgM | ZIKV IgG | DENV IgM | DENV IgG |
|---|---|---|---|---|
| Step 2 (subtraction of cutoff) | ||||
| ZVD (n = 43) | NS1: 29(14) | NS1: 41(39) | NS1: 26(5), WV: 9(1) | NS1: 3(1), WV: 33(30) |
| DF (n = 61) | NS1: 3(0) | NS1: 5(1) | NS1: 57(24), WV:54(19) | NS1: 40(32), WV: 57(53) |
| ZVD/DF discrimination ratio | NS1: 0.67/0.05 = 13.5 (0.33/0) = ∞ | NS1: 0.95/0.08 = 11.6 (0.91/0.016 = 55.5) | NS1: 0.60/0.93 = 0.64 (0.11/0.39 = 0.28) WV: 0.21/0.89 = 0.24 (0.023/0.31 = 0.07) | NS1: 0.0697/0.656 = 0.11 (0.0232/0.524 = 0.04) WV: 0.767/0.934 = 0.82 (0.697/0.869 = 0.80) |
| Per serum, without and with () urea, final score > 1 | ZIKV IgM | ZIKV IgG | DENV IgM | DENV IgG |
|---|---|---|---|---|
| Step 6 (coincidence, average cross-reactivity, avidity) | ||||
| ZVD (n = 43) | 22 (6) | 35 (26) | 0 (0) | 0 (0) |
| DF (n = 61) | 1 (0) | 0 (0) | 39 (3) | 36 (26) |
| ZVD/DF discrimination ratio | 0.51/0.016 = 32.0 (0.14/0 = ∞) | 0.81/0 = ∞ (0.60/0 = ∞) | 0/0.64 = 0 (0/0.05 = 0) | 0/0.59 = 0 (0/0.43 = 0) |
| Per patient, without urea, at least twofold increase (!) or decline (§) | ZIKV NS1 IgM | ZIKV NS1 IgG | DENV NS1 or WV IgM | DENV NS1 or WV IgG |
|---|---|---|---|---|
| Step 7 (reactivity change) | ||||
| ZVD, n = 13, of which 11 were multiply sampled, (nr with change) | !2 §10 (10) | !7 §2 (9) | !0 §0 (0) | !0 §0 (0) |
| DF, n = 37, of which 12 were multiply sampled, (nr with change) | !0 §0 (0) | !0 §0 (0) | !2 §5 (7) | !8 §1 (9) |
| ZVD/DF discrimination ratio | 0.91/0 = ∞ | 0.82/0 = ∞ | 0/0.58 = 0 | 0/0.75 = 0 |
| Per patient | Primary ZVD | Previous ZVD | Primary DF | Previous DF |
|---|---|---|---|---|
| Step 8 (compound judgment) | ||||
| ZVD (n = 13) | 12 | 1 | 0 | 0 |
| DF (n = 37) | 0 | 0 | 35 | 2 |
| ZVD/DF discrimination ratio | 1/0 = ∞ | 0/1 = 0 | ||
Numbers shown in bold emphasize a major discrimination of ZVD and DF