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Secondary structure analysis based on SRCD data
| Helix (%) | Sheet (%) | Disorder (%) | Tm | |
|---|---|---|---|---|
| WT (20 °C) | 64 ± 2 | 5 ± 1 | 21 ± 2 | 38 °C |
| WT (35 °C) | 61 ± 1 | 5 ± 1 | 21 ± 2 | |
| WT (85 °C) | 37 ± 3 | 21 ± 1 | 32 ± 2 | |
| N225D (20 °C) | 66 ± 2 | 5 ± 2 | 19 ± 1 | 40 °C |
| N225D (35 °C) | 62 ± 1 | 6 ± 2 | 22 ± 2 | |
| N225D (85 °C) | 51 ± 3 | 13 ± 1 | 26 ± 3 |
Tm values are calculated from the curves in Fig. 3c. The ± values indicate one standard deviation between values calculated by different algorithms
