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. 2017 Sep 14;7:11514. doi: 10.1038/s41598-017-11876-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein interaction network mapping in HCC1954 HER2+ cells reveals new GRB2 binding partners. (a) Clonal stable cell lines expressing either 3xFLAG-GFP (30 kDa) or 3xFLAG-GRB2 (28 kDa) fusion proteins were analyzed by Western blot to evaluate transgene expression. (b) FLAG-GRB2 cells were analyzed by immunofluorescence to verify protein localization (scale bar = 10 μm). (c) GRB2 protein interaction network obtained by AP-MS from HCC1954 cells treated or not with a phosphatase inhibitor (PV). Proteins displaying a SAINT score above 0.9 are represented in the network (Supplementary Table S1). Edges represent interactions obtained by AP-MS (bold) or previously reported (narrow).