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. 2017 Sep 14;7:11596. doi: 10.1038/s41598-017-11984-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Actin-activated P_i release from Myo19-3IQ·ADP·P_i. The time course of transient P_i release. Myo19-3IQ·ADP·P_i (0.5 µM) after mixing with 0 (a) or 80 µM (b) F-actin in the presence of 8 µM P_iBiP. Myo19-3IQ·ATP were aged for 300 ms before mixing with F-actin (λ_ex = 436 nm, with 455-nm long-pass emission filter). The solid line represents the best fit to the sum of single exponential (initial burst) and linear (steady state turnover) function. Time course in the absence of the actin appears flat over the same timescale, as P_i release from myosin alone is very slow (0.05 s⁻¹).