Figure 8.

Simulation of the ATPase activity and duty ratio of Myo19-3IQ (A). Simulated steady state [actin]-dependence of the ATPase activity of the Myo19-3IQ. The solid lines through the data points are the best fit to a rectangular hyperbola ($v=v_{0+}(k_{cat}\left[actin\right])/\left(K_m+\left[actin\right]\right))$. (B) Duty ratio as a function of [actin]. The duty ratio was calculated from the summation of all the biochemical intermediates distribution at each [actin] at the steady-state time regime according to equation: duty ratio = (strongly bound states)/(strongly bound states + weakly bound states). The solid lines through the data points are the best fit to a rectangular hyperbola ($dutyratio=1\ast \left[actin\right]/\left(K_{DR}+\left[actin\right]\right)$).