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. 2017 Sep 14;7:11569. doi: 10.1038/s41598-017-11885-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TGF-β1 induces the expression of miR-503 at the transcriptional level. (A) Pri-miR-503 expression was analyzed using quantitative PCR in U251, A172, LN-229 and T98G cells treated with TGF-β1. (B) ChIP-PCR amplification products using primers against the miR-503 promoter region were separated by agarose gel electrophoresis. (C) Evolutionary conservation of the set4 region in miR-503 promoter was shown. (D) qChIP analysis showing the enrichment of Smad2/3 in set 4 in U251, A172, LN-229 and T98G cells. (E) The luciferase reporter assay showing that mut-SBE3 significantly inhibits the increased luciferase activity induced by TGF-β1.