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. 2017 Sep 14;7:11569. doi: 10.1038/s41598-017-11885-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-503 increases glioblastoma cell proliferation and inhibits cell apoptosis. (A) The knockdown or overexpression efficacy of miR-503 was analyzed using quantitative PCR in U251 and A172 transfected with miR-503 inhibitor or miR-503 mimics. (B) The cell proliferation rate was measured with the Alarma Blue assay in cells transfected with miR-503 inhibitor or miR-503 mimics. (C) Colony formation assay of cells transfected with miR-503 inhibitors. (D) Anchorage growth of cells transfected with miR-503 mimics in soft agar. (E) Flow cytometry analysis of the annexin-V positive populations of cells treated with miR-503 mimics..