Figure 5.

Infectivity titers of VSV-RV/CE2E1 in U937 and THP-1 cells stimulated with PMA. U937 and THP-1 were incubated with or without PMA at 37 °C for 72 h and then were infected with the pseudotype viruses. At 24 h p.i. the luciferase activities of the cells were measured. The genome copy numbers in the stock solutions were determined by reverse transcription-quantitative PCR, the RLU per 10¹⁰ copies of the viral genomes are shown. Data represent the mean values ± SD for triplicate samples. The significant differences were determined by two-tailed t-tests. RLU, relative light unit of luciferase activity; ∆G, VSV-∆G; RV/CE2E1, VSV-RV/CE2E1; G, VSV-G.