Figure 5.

Actin activated steady state ATPase activity reveals alterations in Myo19-3IQ^W140V enzymology. The actin filament concentration dependence of the Myo19-3IQ (WT) and Myo19-3IQ ^W140V (W140V) steady-state ATPase activity. The solid line through the data points is the best fit to a rectangular hyperbola ($v=v_{0+}(k_{cat}[actin])/(K_{ATPase}+[actin])$). Error bars represent standard deviation from at least three independent experiments from different protein purifications. Data for Myo19-3IQ (WT) is reproduced from (Usaj & Henn, parallel submission).