Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 14;7:11577. doi: 10.1038/s41598-017-11002-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ATP binding to Acto·Myo19^W140V-3IQ (W140V) shows different kinetic behavior than the Myo19-3IQ (WT) (a). Time courses of light scattering decrease after mixing 0.25 µM actomyosin with 0 µM (a), 1.95 (b), 3.9 (c), 7.8 (d), 62.5 (e), 125(f), µM ATP for Acto·Myo19^W140V-3IQ. Data are averaged transients (n = 3–5). The black lines are the best fits to double exponential function (see SI for analysis of the residuals of the fits). (b) Time course of light scattering decrease after mixing 0.25 µM actomyosin with 0 µM (a) or 7.8 µM ATP for Acto·Myo19-3IQ^W140V (b) or Acto·Myo19-3IQ (c). Data are averaged transients (n = 3–5). The smooth lines through the data represent best fit to double exponential function (c). [ATP]-dependence of the fast-observed rate constant of ATP binding to Acto·Myo19-3IQ^W140V in comparison to Acto·Myo19-3IQ as measured by light scattering. (d) [ATP]-dependence of the slow observed rate constant of ATP binding to Acto·Myo19-3IQ^W140V in comparison to Acto·Myo19-3IQ as measured by light scattering. The lines through the data points in c and d are the best fit to rectangular hyperbola. Error bars of the fitting are within data points. (e) The ratio of the fast (A_fast) to slow (A_slow) amplitudes of the observed fast and slow rate constants as a function of [ATP] for Acto·Myo19-3IQ^W140V in comparison to Acto·Myo19-3IQ. Data for Myo19-3IQ (WT) is reproduced from (Usaj & Henn, parallel submission).