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. 2017 Sep 14;7:11577. doi: 10.1038/s41598-017-11002-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ADP dissociation from Acto·Myo19-3IQ ^W140V (W140V) measured by kinetic competition with ATP in comparison to Acto·Myo19-3IQ (WT). (a) Normalized time courses of light scattering decrease after mixing 0.2 µM Acto·Myo19-3IQ^W140V with 0 µM ATP (upper trace) or 125 µM ATP in presence of different [ADP] ranging from 0 to 25 µM. Data are averaged transients (n = 3–5). Smooth lines (black) through the data represent best fits to double or single exponential functions. (b) [ADP]-dependence of the observed slow rate constant for Acto·Myo19-3IQ and single observed rate constant for Acto·Myo19-3IQ^W140V as measured by kinetic competition with 125 µM ATP. (c) [ADP]-dependence of normalized amplitudes of the slow phase obtained by fitting transients described in (a). (d) [ATP]-dependence of the observed rate constant for Acto·Myo19-3IQ and Acto·Myo19-3IQ^W140V dissociation as measured by light scattering at saturated [ADP] of 25 µM. The lines through the data points in (b,c and d) are the best fits to rectangular hyperbola. Error bars of the fittings are within data points. Data for Myo19-3IQ (WT) is reproduced from (Usaj & Henn, parallel submission).